Advanced Imaging
1: Zhang Z, Kil KE, Poutiainen P, Choi JK, Kang HJ, Huang XP, Roth BL, Brownell AL. Re-exploring the N-phenylpicolinamide derivatives to develop mGlu4 ligands with improved affinity and in vitro microsomal stability. Bioorg Med Chem Lett. 2015 Sep 15;25(18):3956-60. doi: 10.1016/j.bmcl.2015.07.031. Epub 2015 Jul 17. PMID: 26231155; PMCID: PMC4539532.
2: Yuan G, Qu X, Zheng B, Neelamegam R, Afshar S, Iyengar S, Pan C, Wang J, Kang HJ, Ondrechen MJ, Poutiainen P, El Fakhri G, Zhang Z, Brownell AL. Design, Synthesis, and Characterization of Benzimidazole Derivatives as Positron Emission Tomography Imaging Ligands for Metabotropic Glutamate Receptor 2. J Med Chem. 2020 Oct 22;63(20):12060-12072. doi: 10.1021/acs.jmedchem.0c01394. Epub 2020 Oct 7. PMID: 32981322.
3: Ylä-Herttuala S, Hakulinen M, Poutiainen P, Laitinen TM, Koivisto AM, Remes AM, Hallikainen M, Lehtola JM, Saari T, Korhonen V, Könönen M, Vanninen R, Mussalo H, Laitinen T, Mervaala E. Severe Obstructive Sleep Apnea and Increased Cortical Amyloid-β Deposition. J Alzheimers Dis. 2021;79(1):153-161. doi: 10.3233/JAD-200736. PMID: 33216027.
4: Yasmin A, Pitkänen A, Jokivarsi K, Poutiainen P, Gröhn O, Immonen R. MRS Reveals Chronic Inflammation in T2w MRI-Negative Perilesional Cortex - A 6-Months Multimodal Imaging Follow-Up Study. Front Neurosci. 2019 Aug 16;13:863. doi: 10.3389/fnins.2019.00863. PMID: 31474824; PMCID: PMC6707062.
5: Wang J, Qu X, Shoup TM, Yuan G, Afshar S, Pan C, Zhu A, Choi JK, Kang HJ, Poutiainen P, El Fakhri G, Zhang Z, Brownell AL. Synthesis and Characterization of Fluorine-18-Labeled N-(4-Chloro-3-((fluoromethyl-d2)thio)phenyl)picolinamide for Imaging of mGluR4 in Brain. J Med Chem. 2020 Mar 26;63(6):3381-3389. doi: 10.1021/acs.jmedchem.0c00201. Epub 2020 Mar 4. PMID: 32081008; PMCID: PMC7261135.
6: Takalo M, Wittrahm R, Wefers B, Parhizkar S, Jokivarsi K, Kuulasmaa T, Mäkinen P, Martiskainen H, Wurst W, Xiang X, Marttinen M, Poutiainen P, Haapasalo A, Hiltunen M, Haass C. The Alzheimer's disease-associated protective Plcγ2-P522R variant promotes immune functions. Mol Neurodegener. 2020 Sep 11;15(1):52. doi: 10.1186/s13024-020-00402-7. PMID: 32917267; PMCID: PMC7488484.
7: Sundén H, Holland MC, Poutiainen PK, Jääskeläinen T, Pulkkinen JT, Palvimo JJ, Olsson R. Synthesis and biological evaluation of second-generation tropanol-based androgen receptor modulators. J Med Chem. 2015 Feb 12;58(3):1569-74. doi: 10.1021/jm501995n. Epub 2015 Feb 3. PMID: 25646649.
8: Poutiainen P, Jaronen M, Quintana FJ, Brownell AL. Precision Medicine in Multiple Sclerosis: Future of PET Imaging of Inflammation and Reactive Astrocytes. Front Mol Neurosci. 2016 Sep 15;9:85. doi: 10.3389/fnmol.2016.00085. PMID: 27695400; PMCID: PMC5023680.
9: Poutiainen P, Kil KE, Zhang Z, Kuruppu D, Tannous B, Brownell AL. Co-operative binding assay for the characterization of mGlu4 allosteric modulators. Neuropharmacology. 2015 Oct;97:142-8. doi: 10.1016/j.neuropharm.2015.05.017. Epub 2015 May 27. PMID: 26025660; PMCID: PMC4537392.
10: Poutiainen PK, Huhtala T, Jääskeläinen T, Petsalo A, Küblbeck J, Kaikkonen S, Palvimo JJ, Raunio H, Närvänen A, Peräkylä M, Juvonen RO, Honkakoski P, Laatikainen R, Pulkkinen JT. Preclinical pharmacology of FL442, a novel nonsteroidal androgen receptor modulator. Mol Cell Endocrinol. 2014 Apr 25;387(1-2):8-18. doi: 10.1016/j.mce.2014.02.008. Epub 2014 Feb 22. PMID: 24565895.
11: Poutiainen PK, Rönkkö T, Hinkkanen AE, Palvimo JJ, Närvänen A, Turhanen P, Laatikainen R, Weisell J, Pulkkinen JT. Firefly luciferase inhibitor-conjugated peptide quenches bioluminescence: a versatile tool for real time monitoring cellular uptake of biomolecules. Bioconjug Chem. 2014 Jan 15;25(1):4-10. doi: 10.1021/bc4003713. Epub 2013 Dec 19. PMID: 24341748.
12: Poutiainen PK, Palvimo JJ, Hinkkanen AE, Valkonen A, Väisänen TK, Laatikainen R, Pulkkinen JT. Discovery of 5-benzyl-3-phenyl-4,5-dihydroisoxazoles and 5-benzyl-3-phenyl-1,4,2-dioxazoles as potent firefly luciferase inhibitors. J Med Chem. 2013 Feb 14;56(3):1064-73. doi: 10.1021/jm301516q. Epub 2013 Jan 17. PMID: 23286196.
13: Poutiainen PK, Oravilahti T, Peräkylä M, Palvimo JJ, Ihalainen JA, Laatikainen R, Pulkkinen JT. Design, synthesis, and biological evaluation of nonsteroidal cycloalkane[d]isoxazole-containing androgen receptor modulators. J Med Chem. 2012 Jul 26;55(14):6316-27. doi: 10.1021/jm300233k. Epub 2012 Jul 11. PMID: 22746350.
14: Poutiainen PK, Venäläinen TA, Peräkylä M, Matilainen JM, Väisänen S, Honkakoski P, Laatikainen R, Pulkkinen JT. Synthesis and biological evaluation of phenolic 4,5-dihydroisoxazoles and 3-hydroxy ketones as estrogen receptor alpha and beta agonists. Bioorg Med Chem. 2010 May 15;18(10):3437-47. doi: 10.1016/j.bmc.2010.04.007. Epub 2010 Apr 7. PMID: 20430632.
15: Nair RR, Koivisto H, Jokivarsi K, Miinalainen IJ, Autio KJ, Manninen A, Poutiainen P, Tanila H, Hiltunen JK, Kastaniotis AJ. Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice. J Neurosci. 2018 Nov 7;38(45):9781-9800. doi: 10.1523/JNEUROSCI.3514-17.2018. Epub 2018 Sep 28. PMID: 30266742; PMCID: PMC6595986.
16: Laatikainen R, Hassinen T, Lehtivarjo J, Tiainen M, Jungman J, Tynkkynen T, Korhonen SP, Niemitz M, Poutiainen P, Jääskeläinen O, Väisänen T, Weisell J, Soininen P, Laatikainen P, Martonen H, Tuppurainen K. Comprehensive strategy for proton chemical shift prediction: linear prediction with nonlinear corrections. J Chem Inf Model. 2014 Feb 24;54(2):419-30. doi: 10.1021/ci400648s. Epub 2014 Feb 11. PMID: 24455975.
17: Kil KE, Poutiainen P, Zhang Z, Zhu A, Kuruppu D, Prabhakar S, Choi JK, Tannous BA, Brownell AL. Synthesis and evaluation of N-(methylthiophenyl)picolinamide derivatives as PET radioligands for metabotropic glutamate receptor subtype 4. Bioorg Med Chem Lett. 2016 Jan 1;26(1):133-9. doi: 10.1016/j.bmcl.2015.11.015. Epub 2015 Nov 10. PMID: 26602273; PMCID: PMC4684791.
18: Kil KE, Poutiainen P, Zhang Z, Zhu A, Choi JK, Jokivarsi K, Brownell AL. Radiosynthesis and evaluation of an 18F-labeled positron emission tomography (PET) radioligand for metabotropic glutamate receptor subtype 4 (mGlu4). J Med Chem. 2014 Nov 13;57(21):9130-8. doi: 10.1021/jm501245b. Epub 2014 Oct 27. PMID: 25330258; PMCID: PMC4230996.
19: Johnson TB, Brudvig JJ, Lehtimäki KK, Cain JT, White KA, Bragge T, Rytkönen J, Huhtala T, Timm D, Vihma M, Puoliväli JT, Poutiainen P, Nurmi A, Weimer JM. A multimodal approach to identify clinically relevant biomarkers to comprehensively monitor disease progression in a mouse model of pediatric neurodegenerative disease. Prog Neurobiol. 2020 Jun;189:101789. doi: 10.1016/j.pneurobio.2020.101789. Epub 2020 Mar 18. PMID: 32198061.
20: Jenkins BG, Zhu A, Poutiainen P, Choi JK, Kil KE, Zhang Z, Kuruppu D, Aytan N, Dedeoglu A, Brownell AL. Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration. Neuropharmacology. 2016 Sep;108:462-73. doi: 10.1016/j.neuropharm.2015.11.010. Epub 2015 Nov 12. PMID: 26581500; PMCID: PMC4896842.
21: Huhtala T, Poutiainen P, Rytkönen J, Lehtimäki K, Parkkari T, Kasanen I, Airaksinen AJ, Koivula T, Sweeney P, Kontkanen O, Wityak J, Dominiquez C, Park LC. Improved synthesis of [18F] fallypride and characterization of a Huntington's disease mouse model, zQ175DN KI, using longitudinal PET imaging of D2/D3 receptors. EJNMMI Radiopharm Chem. 2019 Aug 5;4(1):20. doi: 10.1186/s41181-019-0071-6. PMID: 31659519; PMCID: PMC6682833.
22: Gabbouj S, Natunen T, Koivisto H, Jokivarsi K, Takalo M, Marttinen M, Wittrahm R, Kemppainen S, Naderi R, Posado-Fernández A, Ryhänen S, Mäkinen P, Paldanius KMA, Doria G, Poutiainen P, Flores O, Haapasalo A, Tanila H, Hiltunen M. Intranasal insulin activates Akt2 signaling pathway in the hippocampus of wild-type but not in APP/PS1 Alzheimer model mice. Neurobiol Aging. 2019 Mar;75:98-108. doi: 10.1016/j.neurobiolaging.2018.11.008. Epub 2018 Nov 17. PMID: 30554086.
23: Brownell AL, Kuruppu D, Kil KE, Jokivarsi K, Poutiainen P, Zhu A, Maxwell M. PET imaging studies show enhanced expression of mGluR5 and inflammatory response during progressive degeneration in ALS mouse model expressing SOD1-G93A gene. J Neuroinflammation. 2015 Nov 24;12:217. doi: 10.1186/s12974-015-0439-9. PMID: 26597638; PMCID: PMC4657381.
24: Arsenault D, Coulombe K, Zhu A, Gong C, Kil KE, Choi JK, Poutiainen P, Brownell AL. Loss of Metabotropic Glutamate Receptor 5 Function on Peripheral Benzodiazepine Receptor in Mice Prenatally Exposed to LPS. PLoS One. 2015 Nov 4;10(11):e0142093. doi: 10.1371/journal.pone.0142093. PMID: 26536027; PMCID: PMC4633140.
25: Silvola JMU, Li XG, Virta J, Marjamäki P, Liljenbäck H, Hytönen JP, Tarkia M, Saunavaara V, Hurme S, Palani S, Hakovirta H, Ylä-Herttuala S, Saukko P, Chen Q, Low PS, Knuuti J, Saraste A, Roivainen A. Aluminum fluoride-18 labeled folate enables in vivo detection of atherosclerotic plaque inflammation by positron emission tomography. Sci Rep. 2018 Jun 26;8(1):9720. doi: 10.1038/s41598-018-27618-4. PMID: 29946129; PMCID: PMC6018703.
26: Korpisalo P, Hytönen JP, Laitinen JT, Närväinen J, Rissanen TT, Gröhn OH, Ylä-Herttuala S. Ultrasound imaging with bolus delivered contrast agent for the detection of angiogenesis and blood flow irregularities. Am J Physiol Heart Circ Physiol. 2014 Oct 15;307(8):H1226-32. doi: 10.1152/ajpheart.00315.2013. Epub 2014 Aug 22. PMID: 25320333.
27: Asano T, Hytönen J, Chichareon P, Taavitsainen J, Kogame N, Katagiri Y, Miyazaki Y, Takahashi K, Modolo R, Komiyama H, Tenekecioglu E, Sotomi Y, Wykrzykowska JJ, Piek JJ, Martin J, Baumbach A, Mathur A, Onuma Y, Ylä-Herttuala S, Serruys PW. Serial Optical Coherence Tomography at Baseline, 7 Days, and 1, 3, 6 and 12 Months After Bioresorbable Scaffold Implantation in a Growing Porcine Model. Circ J. 2019 Feb 25;83(3):556-566. doi: 10.1253/circj.CJ-18-0855. Epub 2019 Jan 31. PMID: 30700665.
Preclinical studies
1: Nurro J, Halonen PJ, Kuivanen A, Tarkia M, Saraste A, Honkonen K, Lähteenvuo J, Rissanen TT, Knuuti J, Ylä-Herttuala S. AdVEGF-B186 and AdVEGF-DΔNΔC induce angiogenesis and increase perfusion in porcine myocardium. Heart. 2016 Nov 1;102(21):1716-1720. doi: 10.1136/heartjnl-2016-309373. Epub 2016 May 12. PMID: 27173504.
2: Pajula JJ, Halonen PJ, Hätinen OP, Ylä-Herttuala S, Nurro J. Adenoviral Gene Transfer of Gremlin Modulates Vascular Endothelial Growth Factor-A-Induced Angiogenesis in Porcine Myocardium. Hum Gene Ther. 2020 Feb;31(3-4):211-218. doi: 10.1089/hum.2019.229. Epub 2020 Jan 28. PMID: 31884825.
3: Lähteenvuo J, Hätinen OP, Kuivanen A, Huusko J, Paananen J, Lähteenvuo M, Nurro J, Hedman M, Hartikainen J, Laham-Karam N, Mäkinen P, Räsänen M, Alitalo K, Rosenzweig A, Ylä-Herttuala S. Susceptibility to Cardiac Arrhythmias and Sympathetic Nerve Growth in VEGF-B Overexpressing Myocardium. Mol Ther. 2020 Jul 8;28(7):1731-1740. doi: 10.1016/j.ymthe.2020.03.011. Epub 2020 Mar 19. PMID: 32243833; PMCID: PMC7335717.
4: Halonen PJ, Nurro J, Kuivanen A, Ylä-Herttuala S. Current gene therapy trials for vascular diseases. Expert Opin Biol Ther. 2014 Mar;14(3):327-36. doi: 10.1517/14712598.2014.872237. Epub 2014 Jan 3. PMID: 24387602.
5: Nowak-Sliwinska P, Alitalo K, Allen E, Anisimov A, Aplin AC, Auerbach R, Augustin HG, Bates DO, van Beijnum JR, Bender RHF, Bergers G, Bikfalvi A, Bischoff J, Böck BC, Brooks PC, Bussolino F, Cakir B, Carmeliet P, Castranova D, Cimpean AM, Cleaver O, Coukos G, Davis GE, De Palma M, Dimberg A, Dings RPM, Djonov V, Dudley AC, Dufton NP, Fendt SM, Ferrara N, Fruttiger M, Fukumura D, Ghesquière B, Gong Y, Griffin RJ, Harris AL, Hughes CCW, Hultgren NW, Iruela-Arispe ML, Irving M, Jain RK, Kalluri R, Kalucka J, Kerbel RS, Kitajewski J, Klaassen I, Kleinmann HK, Koolwijk P, Kuczynski E, Kwak BR, Marien K, Melero-Martin JM, Munn LL, Nicosia RF, Noel A, Nurro J, Olsson AK, Petrova TV, Pietras K, Pili R, Pollard JW, Post MJ, Quax PHA, Rabinovich GA, Raica M, Randi AM, Ribatti D, Ruegg C, Schlingemann RO, Schulte-Merker S, Smith LEH, Song JW, Stacker SA, Stalin J, Stratman AN, Van de Velde M, van Hinsbergh VWM, Vermeulen PB, Waltenberger J, Weinstein BM, Xin H, Yetkin-Arik B, Yla-Herttuala S, Yoder MC, Griffioen AW. Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis. 2018 Aug;21(3):425-532. doi: 10.1007/s10456-018-9613-x. PMID: 29766399; PMCID: PMC6237663.
6: Hätinen OA, Lähteenvuo JE, Korpela HJ, Pajula JJ, Ylä-Herttuala S. Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies. BMC Mol Cell Biol. 2019 Aug 13;20(1):32. doi: 10.1186/s12860-019-0215-2. PMID: 31409295; PMCID: PMC6693209.
7: Korpisalo P, Hytönen JP, Laitinen JT, Laidinen S, Parviainen H, Karvinen H, Siponen J, Marjomäki V, Vajanto I, Rissanen TT, Ylä-Herttuala S. Capillary enlargement, not sprouting angiogenesis, determines beneficial therapeutic effects and side effects of angiogenic gene therapy. Eur Heart J. 2011 Jul;32(13):1664-72. doi: 10.1093/eurheartj/ehq433. Epub 2010 Dec 7. PMID: 21138938.
8: Hytönen J, Leppänen O, Braesen JH, Schunck WH, Mueller D, Jung F, Mrowietz C, Jastroch M, von Bergwelt-Baildon M, Kappert K, Heuser A, Drenckhahn JD, Pieske B, Thierfelder L, Ylä-Herttuala S, Blaschke F. Activation of Peroxisome Proliferator-Activated Receptor-δ as Novel Therapeutic Strategy to Prevent In-Stent Restenosis and Stent Thrombosis. Arterioscler Thromb Vasc Biol. 2016 Aug;36(8):1534-48. doi: 10.1161/ATVBAHA.115.306962. Epub 2016 Jun 9. PMID: 27283742.
9: Hytönen JP, Leppänen O, Taavitsainen J, Korpisalo P, Laidinen S, Alitalo K, Wadström J, Rissanen TT, Ylä-Herttuala S. Improved endothelialization of small-diameter ePTFE vascular grafts through growth factor therapy. Vasc Biol. 2019 Jan 3;1(1):1-9. doi: 10.1530/VB-18-0001. PMID: 32923945; PMCID: PMC7449264.
10: Hytönen JP, Taavitsainen J, Laitinen JTT, Partanen A, Alitalo K, Leppänen O, Ylä-Herttuala S. Local adventitial anti-angiogenic gene therapy reduces growth of vasa-vasorum and in-stent restenosis in WHHL rabbits. J Mol Cell Cardiol. 2018 Aug;121:145-154. doi: 10.1016/j.yjmcc.2018.07.007. Epub 2018 Jul 9. PMID: 30003882.
11: Hytönen JP, Taavitsainen J, Tarvainen S, Ylä-Herttuala S. Biodegradable coronary scaffolds: their future and clinical and technological challenges. Cardiovasc Res. 2018 Jul 1;114(8):1063-1072. doi: 10.1093/cvr/cvy097. PMID: 29718125.
Preclinical models
1: Rissanen TT, Nurro J, Halonen PJ, Tarkia M, Saraste A, Rannankari M, Honkonen K, Pietilä M, Leppänen O, Kuivanen A, Knuuti J, Ylä-Herttuala S. The bottleneck stent model for chronic myocardial ischemia and heart failure in pigs. Am J Physiol Heart Circ Physiol. 2013 Nov 1;305(9):H1297-308. doi: 10.1152/ajpheart.00561.2013. Epub 2013 Aug 30. PMID: 23997096.
2: Taavitsainen J, Tarvainen S, Kuivanen A, Mangiardi EK, Guelcher M, Martin J, Mathur A, Hytönen JP, Ylä-Herttuala S. Evaluation of Biodegradable Stent Graft Coatings in Pig and Rabbit Models. J Vasc Res. 2020;57(2):65-75. doi: 10.1159/000505454. Epub 2020 Feb 7. PMID: 32036370.
1: Zhang Z, Kil KE, Poutiainen P, Choi JK, Kang HJ, Huang XP, Roth BL, Brownell AL. Re-exploring the N-phenylpicolinamide derivatives to develop mGlu4 ligands with improved affinity and in vitro microsomal stability. Bioorg Med Chem Lett. 2015 Sep 15;25(18):3956-60. doi: 10.1016/j.bmcl.2015.07.031. Epub 2015 Jul 17. PMID: 26231155; PMCID: PMC4539532.
2: Yuan G, Qu X, Zheng B, Neelamegam R, Afshar S, Iyengar S, Pan C, Wang J, Kang HJ, Ondrechen MJ, Poutiainen P, El Fakhri G, Zhang Z, Brownell AL. Design, Synthesis, and Characterization of Benzimidazole Derivatives as Positron Emission Tomography Imaging Ligands for Metabotropic Glutamate Receptor 2. J Med Chem. 2020 Oct 22;63(20):12060-12072. doi: 10.1021/acs.jmedchem.0c01394. Epub 2020 Oct 7. PMID: 32981322.
3: Ylä-Herttuala S, Hakulinen M, Poutiainen P, Laitinen TM, Koivisto AM, Remes AM, Hallikainen M, Lehtola JM, Saari T, Korhonen V, Könönen M, Vanninen R, Mussalo H, Laitinen T, Mervaala E. Severe Obstructive Sleep Apnea and Increased Cortical Amyloid-β Deposition. J Alzheimers Dis. 2021;79(1):153-161. doi: 10.3233/JAD-200736. PMID: 33216027.
4: Yasmin A, Pitkänen A, Jokivarsi K, Poutiainen P, Gröhn O, Immonen R. MRS Reveals Chronic Inflammation in T2w MRI-Negative Perilesional Cortex - A 6-Months Multimodal Imaging Follow-Up Study. Front Neurosci. 2019 Aug 16;13:863. doi: 10.3389/fnins.2019.00863. PMID: 31474824; PMCID: PMC6707062.
5: Wang J, Qu X, Shoup TM, Yuan G, Afshar S, Pan C, Zhu A, Choi JK, Kang HJ, Poutiainen P, El Fakhri G, Zhang Z, Brownell AL. Synthesis and Characterization of Fluorine-18-Labeled N-(4-Chloro-3-((fluoromethyl-d2)thio)phenyl)picolinamide for Imaging of mGluR4 in Brain. J Med Chem. 2020 Mar 26;63(6):3381-3389. doi: 10.1021/acs.jmedchem.0c00201. Epub 2020 Mar 4. PMID: 32081008; PMCID: PMC7261135.
6: Takalo M, Wittrahm R, Wefers B, Parhizkar S, Jokivarsi K, Kuulasmaa T, Mäkinen P, Martiskainen H, Wurst W, Xiang X, Marttinen M, Poutiainen P, Haapasalo A, Hiltunen M, Haass C. The Alzheimer's disease-associated protective Plcγ2-P522R variant promotes immune functions. Mol Neurodegener. 2020 Sep 11;15(1):52. doi: 10.1186/s13024-020-00402-7. PMID: 32917267; PMCID: PMC7488484.
7: Sundén H, Holland MC, Poutiainen PK, Jääskeläinen T, Pulkkinen JT, Palvimo JJ, Olsson R. Synthesis and biological evaluation of second-generation tropanol-based androgen receptor modulators. J Med Chem. 2015 Feb 12;58(3):1569-74. doi: 10.1021/jm501995n. Epub 2015 Feb 3. PMID: 25646649.
8: Poutiainen P, Jaronen M, Quintana FJ, Brownell AL. Precision Medicine in Multiple Sclerosis: Future of PET Imaging of Inflammation and Reactive Astrocytes. Front Mol Neurosci. 2016 Sep 15;9:85. doi: 10.3389/fnmol.2016.00085. PMID: 27695400; PMCID: PMC5023680.
9: Poutiainen P, Kil KE, Zhang Z, Kuruppu D, Tannous B, Brownell AL. Co-operative binding assay for the characterization of mGlu4 allosteric modulators. Neuropharmacology. 2015 Oct;97:142-8. doi: 10.1016/j.neuropharm.2015.05.017. Epub 2015 May 27. PMID: 26025660; PMCID: PMC4537392.
10: Poutiainen PK, Huhtala T, Jääskeläinen T, Petsalo A, Küblbeck J, Kaikkonen S, Palvimo JJ, Raunio H, Närvänen A, Peräkylä M, Juvonen RO, Honkakoski P, Laatikainen R, Pulkkinen JT. Preclinical pharmacology of FL442, a novel nonsteroidal androgen receptor modulator. Mol Cell Endocrinol. 2014 Apr 25;387(1-2):8-18. doi: 10.1016/j.mce.2014.02.008. Epub 2014 Feb 22. PMID: 24565895.
11: Poutiainen PK, Rönkkö T, Hinkkanen AE, Palvimo JJ, Närvänen A, Turhanen P, Laatikainen R, Weisell J, Pulkkinen JT. Firefly luciferase inhibitor-conjugated peptide quenches bioluminescence: a versatile tool for real time monitoring cellular uptake of biomolecules. Bioconjug Chem. 2014 Jan 15;25(1):4-10. doi: 10.1021/bc4003713. Epub 2013 Dec 19. PMID: 24341748.
12: Poutiainen PK, Palvimo JJ, Hinkkanen AE, Valkonen A, Väisänen TK, Laatikainen R, Pulkkinen JT. Discovery of 5-benzyl-3-phenyl-4,5-dihydroisoxazoles and 5-benzyl-3-phenyl-1,4,2-dioxazoles as potent firefly luciferase inhibitors. J Med Chem. 2013 Feb 14;56(3):1064-73. doi: 10.1021/jm301516q. Epub 2013 Jan 17. PMID: 23286196.
13: Poutiainen PK, Oravilahti T, Peräkylä M, Palvimo JJ, Ihalainen JA, Laatikainen R, Pulkkinen JT. Design, synthesis, and biological evaluation of nonsteroidal cycloalkane[d]isoxazole-containing androgen receptor modulators. J Med Chem. 2012 Jul 26;55(14):6316-27. doi: 10.1021/jm300233k. Epub 2012 Jul 11. PMID: 22746350.
14: Poutiainen PK, Venäläinen TA, Peräkylä M, Matilainen JM, Väisänen S, Honkakoski P, Laatikainen R, Pulkkinen JT. Synthesis and biological evaluation of phenolic 4,5-dihydroisoxazoles and 3-hydroxy ketones as estrogen receptor alpha and beta agonists. Bioorg Med Chem. 2010 May 15;18(10):3437-47. doi: 10.1016/j.bmc.2010.04.007. Epub 2010 Apr 7. PMID: 20430632.
15: Nair RR, Koivisto H, Jokivarsi K, Miinalainen IJ, Autio KJ, Manninen A, Poutiainen P, Tanila H, Hiltunen JK, Kastaniotis AJ. Impaired Mitochondrial Fatty Acid Synthesis Leads to Neurodegeneration in Mice. J Neurosci. 2018 Nov 7;38(45):9781-9800. doi: 10.1523/JNEUROSCI.3514-17.2018. Epub 2018 Sep 28. PMID: 30266742; PMCID: PMC6595986.
16: Laatikainen R, Hassinen T, Lehtivarjo J, Tiainen M, Jungman J, Tynkkynen T, Korhonen SP, Niemitz M, Poutiainen P, Jääskeläinen O, Väisänen T, Weisell J, Soininen P, Laatikainen P, Martonen H, Tuppurainen K. Comprehensive strategy for proton chemical shift prediction: linear prediction with nonlinear corrections. J Chem Inf Model. 2014 Feb 24;54(2):419-30. doi: 10.1021/ci400648s. Epub 2014 Feb 11. PMID: 24455975.
17: Kil KE, Poutiainen P, Zhang Z, Zhu A, Kuruppu D, Prabhakar S, Choi JK, Tannous BA, Brownell AL. Synthesis and evaluation of N-(methylthiophenyl)picolinamide derivatives as PET radioligands for metabotropic glutamate receptor subtype 4. Bioorg Med Chem Lett. 2016 Jan 1;26(1):133-9. doi: 10.1016/j.bmcl.2015.11.015. Epub 2015 Nov 10. PMID: 26602273; PMCID: PMC4684791.
18: Kil KE, Poutiainen P, Zhang Z, Zhu A, Choi JK, Jokivarsi K, Brownell AL. Radiosynthesis and evaluation of an 18F-labeled positron emission tomography (PET) radioligand for metabotropic glutamate receptor subtype 4 (mGlu4). J Med Chem. 2014 Nov 13;57(21):9130-8. doi: 10.1021/jm501245b. Epub 2014 Oct 27. PMID: 25330258; PMCID: PMC4230996.
19: Johnson TB, Brudvig JJ, Lehtimäki KK, Cain JT, White KA, Bragge T, Rytkönen J, Huhtala T, Timm D, Vihma M, Puoliväli JT, Poutiainen P, Nurmi A, Weimer JM. A multimodal approach to identify clinically relevant biomarkers to comprehensively monitor disease progression in a mouse model of pediatric neurodegenerative disease. Prog Neurobiol. 2020 Jun;189:101789. doi: 10.1016/j.pneurobio.2020.101789. Epub 2020 Mar 18. PMID: 32198061.
20: Jenkins BG, Zhu A, Poutiainen P, Choi JK, Kil KE, Zhang Z, Kuruppu D, Aytan N, Dedeoglu A, Brownell AL. Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration. Neuropharmacology. 2016 Sep;108:462-73. doi: 10.1016/j.neuropharm.2015.11.010. Epub 2015 Nov 12. PMID: 26581500; PMCID: PMC4896842.
21: Huhtala T, Poutiainen P, Rytkönen J, Lehtimäki K, Parkkari T, Kasanen I, Airaksinen AJ, Koivula T, Sweeney P, Kontkanen O, Wityak J, Dominiquez C, Park LC. Improved synthesis of [18F] fallypride and characterization of a Huntington's disease mouse model, zQ175DN KI, using longitudinal PET imaging of D2/D3 receptors. EJNMMI Radiopharm Chem. 2019 Aug 5;4(1):20. doi: 10.1186/s41181-019-0071-6. PMID: 31659519; PMCID: PMC6682833.
22: Gabbouj S, Natunen T, Koivisto H, Jokivarsi K, Takalo M, Marttinen M, Wittrahm R, Kemppainen S, Naderi R, Posado-Fernández A, Ryhänen S, Mäkinen P, Paldanius KMA, Doria G, Poutiainen P, Flores O, Haapasalo A, Tanila H, Hiltunen M. Intranasal insulin activates Akt2 signaling pathway in the hippocampus of wild-type but not in APP/PS1 Alzheimer model mice. Neurobiol Aging. 2019 Mar;75:98-108. doi: 10.1016/j.neurobiolaging.2018.11.008. Epub 2018 Nov 17. PMID: 30554086.
23: Brownell AL, Kuruppu D, Kil KE, Jokivarsi K, Poutiainen P, Zhu A, Maxwell M. PET imaging studies show enhanced expression of mGluR5 and inflammatory response during progressive degeneration in ALS mouse model expressing SOD1-G93A gene. J Neuroinflammation. 2015 Nov 24;12:217. doi: 10.1186/s12974-015-0439-9. PMID: 26597638; PMCID: PMC4657381.
24: Arsenault D, Coulombe K, Zhu A, Gong C, Kil KE, Choi JK, Poutiainen P, Brownell AL. Loss of Metabotropic Glutamate Receptor 5 Function on Peripheral Benzodiazepine Receptor in Mice Prenatally Exposed to LPS. PLoS One. 2015 Nov 4;10(11):e0142093. doi: 10.1371/journal.pone.0142093. PMID: 26536027; PMCID: PMC4633140.
25: Silvola JMU, Li XG, Virta J, Marjamäki P, Liljenbäck H, Hytönen JP, Tarkia M, Saunavaara V, Hurme S, Palani S, Hakovirta H, Ylä-Herttuala S, Saukko P, Chen Q, Low PS, Knuuti J, Saraste A, Roivainen A. Aluminum fluoride-18 labeled folate enables in vivo detection of atherosclerotic plaque inflammation by positron emission tomography. Sci Rep. 2018 Jun 26;8(1):9720. doi: 10.1038/s41598-018-27618-4. PMID: 29946129; PMCID: PMC6018703.
26: Korpisalo P, Hytönen JP, Laitinen JT, Närväinen J, Rissanen TT, Gröhn OH, Ylä-Herttuala S. Ultrasound imaging with bolus delivered contrast agent for the detection of angiogenesis and blood flow irregularities. Am J Physiol Heart Circ Physiol. 2014 Oct 15;307(8):H1226-32. doi: 10.1152/ajpheart.00315.2013. Epub 2014 Aug 22. PMID: 25320333.
27: Asano T, Hytönen J, Chichareon P, Taavitsainen J, Kogame N, Katagiri Y, Miyazaki Y, Takahashi K, Modolo R, Komiyama H, Tenekecioglu E, Sotomi Y, Wykrzykowska JJ, Piek JJ, Martin J, Baumbach A, Mathur A, Onuma Y, Ylä-Herttuala S, Serruys PW. Serial Optical Coherence Tomography at Baseline, 7 Days, and 1, 3, 6 and 12 Months After Bioresorbable Scaffold Implantation in a Growing Porcine Model. Circ J. 2019 Feb 25;83(3):556-566. doi: 10.1253/circj.CJ-18-0855. Epub 2019 Jan 31. PMID: 30700665.
Preclinical studies
1: Nurro J, Halonen PJ, Kuivanen A, Tarkia M, Saraste A, Honkonen K, Lähteenvuo J, Rissanen TT, Knuuti J, Ylä-Herttuala S. AdVEGF-B186 and AdVEGF-DΔNΔC induce angiogenesis and increase perfusion in porcine myocardium. Heart. 2016 Nov 1;102(21):1716-1720. doi: 10.1136/heartjnl-2016-309373. Epub 2016 May 12. PMID: 27173504.
2: Pajula JJ, Halonen PJ, Hätinen OP, Ylä-Herttuala S, Nurro J. Adenoviral Gene Transfer of Gremlin Modulates Vascular Endothelial Growth Factor-A-Induced Angiogenesis in Porcine Myocardium. Hum Gene Ther. 2020 Feb;31(3-4):211-218. doi: 10.1089/hum.2019.229. Epub 2020 Jan 28. PMID: 31884825.
3: Lähteenvuo J, Hätinen OP, Kuivanen A, Huusko J, Paananen J, Lähteenvuo M, Nurro J, Hedman M, Hartikainen J, Laham-Karam N, Mäkinen P, Räsänen M, Alitalo K, Rosenzweig A, Ylä-Herttuala S. Susceptibility to Cardiac Arrhythmias and Sympathetic Nerve Growth in VEGF-B Overexpressing Myocardium. Mol Ther. 2020 Jul 8;28(7):1731-1740. doi: 10.1016/j.ymthe.2020.03.011. Epub 2020 Mar 19. PMID: 32243833; PMCID: PMC7335717.
4: Halonen PJ, Nurro J, Kuivanen A, Ylä-Herttuala S. Current gene therapy trials for vascular diseases. Expert Opin Biol Ther. 2014 Mar;14(3):327-36. doi: 10.1517/14712598.2014.872237. Epub 2014 Jan 3. PMID: 24387602.
5: Nowak-Sliwinska P, Alitalo K, Allen E, Anisimov A, Aplin AC, Auerbach R, Augustin HG, Bates DO, van Beijnum JR, Bender RHF, Bergers G, Bikfalvi A, Bischoff J, Böck BC, Brooks PC, Bussolino F, Cakir B, Carmeliet P, Castranova D, Cimpean AM, Cleaver O, Coukos G, Davis GE, De Palma M, Dimberg A, Dings RPM, Djonov V, Dudley AC, Dufton NP, Fendt SM, Ferrara N, Fruttiger M, Fukumura D, Ghesquière B, Gong Y, Griffin RJ, Harris AL, Hughes CCW, Hultgren NW, Iruela-Arispe ML, Irving M, Jain RK, Kalluri R, Kalucka J, Kerbel RS, Kitajewski J, Klaassen I, Kleinmann HK, Koolwijk P, Kuczynski E, Kwak BR, Marien K, Melero-Martin JM, Munn LL, Nicosia RF, Noel A, Nurro J, Olsson AK, Petrova TV, Pietras K, Pili R, Pollard JW, Post MJ, Quax PHA, Rabinovich GA, Raica M, Randi AM, Ribatti D, Ruegg C, Schlingemann RO, Schulte-Merker S, Smith LEH, Song JW, Stacker SA, Stalin J, Stratman AN, Van de Velde M, van Hinsbergh VWM, Vermeulen PB, Waltenberger J, Weinstein BM, Xin H, Yetkin-Arik B, Yla-Herttuala S, Yoder MC, Griffioen AW. Consensus guidelines for the use and interpretation of angiogenesis assays. Angiogenesis. 2018 Aug;21(3):425-532. doi: 10.1007/s10456-018-9613-x. PMID: 29766399; PMCID: PMC6237663.
6: Hätinen OA, Lähteenvuo JE, Korpela HJ, Pajula JJ, Ylä-Herttuala S. Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies. BMC Mol Cell Biol. 2019 Aug 13;20(1):32. doi: 10.1186/s12860-019-0215-2. PMID: 31409295; PMCID: PMC6693209.
7: Korpisalo P, Hytönen JP, Laitinen JT, Laidinen S, Parviainen H, Karvinen H, Siponen J, Marjomäki V, Vajanto I, Rissanen TT, Ylä-Herttuala S. Capillary enlargement, not sprouting angiogenesis, determines beneficial therapeutic effects and side effects of angiogenic gene therapy. Eur Heart J. 2011 Jul;32(13):1664-72. doi: 10.1093/eurheartj/ehq433. Epub 2010 Dec 7. PMID: 21138938.
8: Hytönen J, Leppänen O, Braesen JH, Schunck WH, Mueller D, Jung F, Mrowietz C, Jastroch M, von Bergwelt-Baildon M, Kappert K, Heuser A, Drenckhahn JD, Pieske B, Thierfelder L, Ylä-Herttuala S, Blaschke F. Activation of Peroxisome Proliferator-Activated Receptor-δ as Novel Therapeutic Strategy to Prevent In-Stent Restenosis and Stent Thrombosis. Arterioscler Thromb Vasc Biol. 2016 Aug;36(8):1534-48. doi: 10.1161/ATVBAHA.115.306962. Epub 2016 Jun 9. PMID: 27283742.
9: Hytönen JP, Leppänen O, Taavitsainen J, Korpisalo P, Laidinen S, Alitalo K, Wadström J, Rissanen TT, Ylä-Herttuala S. Improved endothelialization of small-diameter ePTFE vascular grafts through growth factor therapy. Vasc Biol. 2019 Jan 3;1(1):1-9. doi: 10.1530/VB-18-0001. PMID: 32923945; PMCID: PMC7449264.
10: Hytönen JP, Taavitsainen J, Laitinen JTT, Partanen A, Alitalo K, Leppänen O, Ylä-Herttuala S. Local adventitial anti-angiogenic gene therapy reduces growth of vasa-vasorum and in-stent restenosis in WHHL rabbits. J Mol Cell Cardiol. 2018 Aug;121:145-154. doi: 10.1016/j.yjmcc.2018.07.007. Epub 2018 Jul 9. PMID: 30003882.
11: Hytönen JP, Taavitsainen J, Tarvainen S, Ylä-Herttuala S. Biodegradable coronary scaffolds: their future and clinical and technological challenges. Cardiovasc Res. 2018 Jul 1;114(8):1063-1072. doi: 10.1093/cvr/cvy097. PMID: 29718125.
Preclinical models
1: Rissanen TT, Nurro J, Halonen PJ, Tarkia M, Saraste A, Rannankari M, Honkonen K, Pietilä M, Leppänen O, Kuivanen A, Knuuti J, Ylä-Herttuala S. The bottleneck stent model for chronic myocardial ischemia and heart failure in pigs. Am J Physiol Heart Circ Physiol. 2013 Nov 1;305(9):H1297-308. doi: 10.1152/ajpheart.00561.2013. Epub 2013 Aug 30. PMID: 23997096.
2: Taavitsainen J, Tarvainen S, Kuivanen A, Mangiardi EK, Guelcher M, Martin J, Mathur A, Hytönen JP, Ylä-Herttuala S. Evaluation of Biodegradable Stent Graft Coatings in Pig and Rabbit Models. J Vasc Res. 2020;57(2):65-75. doi: 10.1159/000505454. Epub 2020 Feb 7. PMID: 32036370.